Real-Time PCR Assay for Differentiation of Typhoidal and Nontyphoidal Salmonella

AUTHORS

Satheesh Nair, Vineet Patel, Tadgh Hickey, Clare Maguire, David R Greig, Winnie Lee, Gauri Godbole, Kathie Grant, Marie Anne Chattaway

ABSTRACT

Rapid and accurate differentiation of Salmonella spp. causing enteric fever from nontyphoidal Salmonella is essential for clinical management of cases, laboratory risk management, and implementation of public health measures. Current methods used for confirmation of identification, including biochemistry and serotyping as well as whole-genome sequencing analyses, take several days. Here we report the development and evaluation of a real-time PCR assay that can be performed directly on crude DNA extracts from bacterial colonies for the rapid identification of typhoidal and nontyphoidal Salmonella.

Click here to read the article, published in the Journal of Clinical Microbiology.